论文部分内容阅读
应用合酶链反应（ＰＣＲ），核酸分子杂交和病毒分离技术检测了７例临床诊断为病毒性脑炎患者及１０例其他神经疾病对照组患者的脑脊液（ＣＳＦ）中单纯疱疹病毒（ＨＳＶ）ＤＮＡ。结果ＰＣＲ检出５例脑炎患者ＣＳＦ中ＨＳＶＤＮＡ阳性，其中ＨＳＶ１型（ＨＳＶ－１）２例，ＨＳＶ２型（ＨＳＶ－２）３例，而核酸杂交方法仅检出２例，其中ＨＳＶ－１和ＨＳＶ－２各１例，与病毒分离法结果一致。ＰＣＲ技术在无菌性脑炎的诊断上具有更高的敏感性和特异性，因而较为实用。实验还比较了２种ＤＮＡ模板制备方法，煮沸法简便快速，更宜推广。
The herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF) of 7 clinically diagnosed patients with viral encephalitis and 10 controls of other neurological diseases was detected by polymerase chain reaction (PCR), nucleic acid hybridization and virus isolation techniques . Results HSV-1 was detected in CSF of 5 encephalitis patients, including 2 HSV-1 (HSV-1) and 3 HSV-2 HSV-2, and only 2 HSV- HSV-2 in 1 case, consistent with the results of virus isolation. PCR technology in the diagnosis of aseptic encephalitis with higher sensitivity and specificity, which is more practical. The experiment also compared the preparation of two kinds of DNA template, boiled method is simple and quick, more suitable to promote.