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流式细胞术(flow cytometry,FCM)自70年代中期至今仍是研究细胞生物学等物质的重要技术,已被广泛应用于肿瘤学、免疫学、血液学、药物学等领域。它具有灵敏度高、准确性好、特异性强、重复性好的优点。本文总结了应用流式细胞仪检测353例肝癌患者外周血淋巴细胞CD4+ CD25+ 、CD8+ CD25+ 含量的结果,显示肝癌患者外周血淋巴细胞CD25+ CD4+ 、CD25CD8+ 的百分比低于正常参考值(P＜0.05),有显著性差异。
Flow cytometry (FCM) has been an important technique for studying cellular biology since the mid 1970s and has been widely used in oncology, immunology, hematology and pharmacology. It has the advantages of high sensitivity, good accuracy, strong specificity and good repeatability. This article summarizes the results of flow cytometry in detecting peripheral blood lymphocyte CD4 + CD25 + and CD8 + CD25 + in 353 patients with hepatocellular carcinoma. The percentage of CD25 + CD4 + and CD25 + CD8 + of peripheral blood lymphocytes in patients with hepatocellular carcinoma is lower than the normal reference value (P <0.05) There are significant differences.