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目的:研究肺表面活性物质(PS)对豚鼠分泌性中耳炎(OME)的治疗作用,为OME的治疗探讨新途径。方法:将50只豚鼠随机分为对照组(10只)和实验组(40只),实验组豚鼠采用灭活的肺炎链球菌(KHP)鼓室内注射,建立OME的动物模型(听力下降,中耳积液);模型建立后再将其分为造模组(20耳)、造模对照组(10耳)、治疗组(24耳,向中耳腔注射0.05mlPS)和未治组(23耳,不做任何治疗)。造模5d后,耳内镜下观察鼓膜及鼓室积液的情况,检测各组豚鼠听性脑干反应(ABR)Ⅰ、Ⅲ波潜伏期及反应阈。结果:造模组豚鼠鼓室内注射KHP悬浮液5d后,鼓室出现积液,鼓膜浑浊,光锥消失;ABR反应阈由对照组的(14.00±3.08)dB提高至(45.00±5.67)dB,其差异有统计学意义(P<0.01);治疗组鼓室内注射PS7d后,鼓室积液减少或消失,反应阈由(45.00±5.67)dB降低至(23.53±6.32)dB,分别与造模组及未治组比较,差异有统计学意义(均P<0.01);未治组鼓室积液无变化,鼓膜更加浑浊,反应阈由(45.00±5.67)dB提高至(65.50±6.85)dB。结论:PS对豚鼠OME有治疗作用。
Objective: To investigate the therapeutic effect of pulmonary surfactant (PS) on secretory otitis media (OME) in guinea pigs and explore new ways for the treatment of OME. Methods: Fifty guinea pigs were randomly divided into control group (n = 10) and experimental group (n = 40). Guinea pigs in experimental group were given intratracheal injection of inactivated S. pneumoniae (KHP) (20 ears), model control group (10 ears), treatment group (24 ears, middle ear cavity injection 0.05mlPS), and untreated group (23 ears) Ear, without any treatment). After modeling for 5 days, the tympanic membrane and tympanic effusion were observed under ear endoscopy. The latency and response threshold of wave Ⅰ and Ⅲ of auditory brainstem response (ABR) in guinea pigs were detected. Results: The tympanic effusion appeared and the tympanic membrane turbid and the light cone disappeared. The ABR threshold increased from (14.00 ± 3.08) dB to (45.00 ± 5.67) dB in the control group (P <0.01). After intratracheal instillation of PS7d in the treatment group, the tympanic effusion decreased or disappeared, and the response threshold decreased from (45.00 ± 5.67) dB to (23.53 ± 6.32) dB, respectively, The difference was statistically significant (all P <0.01). The tympanic effusion of the untreated group was unchanged and the tympanic membrane was cloudy. The response threshold increased from (45.00 ± 5.67) dB to (65.50 ± 6.85) dB. Conclusion: PS has a therapeutic effect on OME in guinea pigs.