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本研究依据Gen Bank数据库中发表的枯草芽孢杆菌cspB基因序列进行优化,人工合成了cspB基因序列,构建植物表达载体,通过农杆菌介导法将cspB基因转入玉米自交系Hi-Ⅱ中,获得转cspB基因玉米材料,经过分子检测获得4个阳性转化事件。在4个浓度水平的NaCl溶液(0,40 mmol/L,80 mmol/L,120 mmol/L)和Na_2CO_3溶液(0,10 mmol/L,20 mmol/L,30 mmol/L)胁迫下,研究其耐盐碱性。结果表明:80 mmol/L的NaCl溶液和20 mmol/L Na_2CO_3溶液可以作为转cspB基因玉米的耐盐碱分析条件;cspB基因已经整合到玉米基因组中,转cspB基因玉米的耐盐碱性得到了显著提高。转cspB基因玉米丰富了耐盐碱玉米种质资源。
This study was based on GenBank database published Bacillus subtilis cspB gene sequence optimization, artificial cspB gene sequence, the construction of plant expression vector, the cspB gene transferred into the maize inbred line Hi-Ⅱ by Agrobacterium-mediated method, The cspB gene maize material was obtained, and 4 positive transformation events were obtained after molecular detection. Under the stress of 4 concentrations of NaCl solution (0,40 mmol / L, 80 mmol / L, 120 mmol / L) and Na2CO3 solution (0,10 mmol / L, 20 mmol / L, 30 mmol / L) Study its salt and alkaline resistance. The results showed that 80 mmol / L NaCl solution and 20 mmol / L Na_2CO_3 solution could be used as a salt tolerance base for the cspB transgenic maize. The cspB gene was integrated into the maize genome and the salinity tolerance of cspB transgenic maize was obtained Significantly increased. Turn cspB gene corn to enrich salt-tolerant corn germplasm resources.