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以内蒙古锡林郭勒草原一株野生蘑菇为试材,采用形态特征和生态习性鉴定及分子鉴定的方法,对该蘑菇的菌丝体基因组DNA进行18S和ITS片段的扩增、测序,测序结果经Blast比对,从而对该蘑菇进行分离鉴定。结果表明:该野生真菌属于白桩菇属Leucopaxillus。另外,通过优化的MS平板培养基避光培养,在MS平板培养基上有原基形成,为日后对该菌种的驯化与栽培提供材料,为其开发与利用奠定基础。其ITS序列已上传GenBank,登录号为KY173356。
A strain of wild mushroom from Xilinguole grassland in Inner Mongolia was used as experimental material. 18S and ITS fragments of mycelium genomic DNA were amplified and sequenced by morphological, ecological identification and molecular identification methods. The results of sequencing were compared by Blast ratio Right, so that the mushroom separation and identification. The results showed that the wild fungus belonged to Leucopaxillus. In addition, through the optimization of MS plate medium dark culture, the formation of primordia on MS plate medium for the domestication and cultivation of the species in the future to provide material for its development and utilization of the foundation. Its ITS sequence has been uploaded GenBank, accession number KY173356.