论文部分内容阅读
论述了液相色谱/质谱联用法(LC-MS)的产生、发展、现状及方向。讨论了联用的可能性、障碍、解决方案及各种联用方式等问题。液相色谱、质谱及气相色谱/质谱联用等方法各具局限性;同时,有机化学、生化、医药、石油化工、环境及食品化学等许多领域又需对大量高分子量、难挥发和热不稳定有机化合物进行分析。这两方面是LC-MS产生和发展的原由。实现LC-MS较GC-MS更为困难,主要由于LC的大量溶剂的去除、LC与MS间压力和流量的巨大差异、样品组分的汽化及LC溶剂的干扰等。上述问题的解决,应从LC、MS及其接口三方面着手。如同GC-MS发展历程一样,LC-MS目前主要致力于各种接口的探讨。理想接口的设计与实施是一种艰巨的尝试。当然也要进一步寻求各种有效的电离方式。然而,事实上近年来LC-MS的发展速度很慢,显示出联用的困难。虽然已出现多种型式(且有数种商品接口出售),但却仍没有令人满意的联用方式,有待于进一步加强研制工作。最后,汇总了现有的十余种接口的简略结构性能、附以示意图,并分析比较了其特点。
The production, development, current situation and direction of liquid chromatography-mass spectrometry (LC-MS) were discussed. Discussed the possibility of joint use, obstacles, solutions and various combinations and other issues. Liquid chromatography, mass spectrometry and gas chromatography / mass spectrometry and other methods have their own limitations; the same time, many areas of organic chemistry, biochemistry, medicine, petrochemicals, environment and food chemistry need a large number of high molecular weight, volatile and thermal Stable organic compounds for analysis. These two aspects are the origin and development of LC-MS. It is more difficult to achieve LC-MS than GC-MS due to the large amount of solvent removed in LC, large differences in pressure and flow between LC and MS, vaporization of sample components and interference with LC solvents. The solution to the above problems should be from LC, MS and its interface three aspects. Like the history of GC-MS, LC-MS is currently focused on a variety of interfaces. The design and implementation of the ideal interface is a daunting attempt. Of course, we must further seek various ways of ionization. However, in fact, the development of LC-MS has been slow in recent years, showing the difficulty of combination. Although there have been many types (and several kinds of commodity interfaces for sale), they are still not satisfactorily used in combination, and the research and development work needs to be further strengthened. Finally, the summary of the existing structure of more than ten kinds of interface performance, attached to the schematic, and analysis of its characteristics.