Various behaviors of cancer cells are strongly influenced by their interaction with extracellular matrices (ECM). We investigate how this interaction may be influenced if the cancer cells’ ability of secreting matrix metalloproteinases (MMPs) to degrade ECM is inhibited by adding the MMP inhibitor. We use MDA-MB-231-GFP cells as model cells and use matrigel to mimic ECM. It is found that the added MMP inhibitor significantly reduces the migration speed of cancer cells covered by matrigel but has little influence on the migration persistence and shape factor of the cells and that with the MMP inhibitor added the presence of matrigel on the top has no influence on the migration speed of the cells but increases the cells’ shape factor and migration persistence.