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采用土培试验和１５Ｎ示踪技术研究了高产施肥条件下４个时期不同施氮量与水稻糙米蛋白质含量的关系。结果表明，孕穗期施氮最有利于糙米蛋白质的积累，糙米１５Ｎ蛋白氮占施用１５Ｎ的比例达 ３６． １５％，插植前施氮次之，分期和乳熟期施氮效果较差。当其它 ３个时期施氮量一定时，某个时期的１５Ｎ施量与糙米１５Ｎ蛋白氮含量呈极显著的直线相关，但４个时期的施氮总量与糙米蛋白氮总量却呈一元二次抛物线关系，其原因是过量施氮使糙米产量和糙米中蛋白质含量下降。
Soil culture experiments and 15N tracing techniques were used to study the relationship between different nitrogen application rates and protein content of rice brown rice during the four periods of high yield fertilization. The results showed that nitrogen application at the booting stage was the most conducive to the accumulation of brown rice protein, and the ratio of 15N protein nitrogen in brown rice to 15N was 36. 15%, followed by nitrogen application before planting, and poor nitrogen application efficiency during staging and milk ripening period. When the nitrogen application rate was constant for the other three periods, there was a significant linear correlation between 15N application and 15N protein nitrogen content in brown rice in a certain period, but the total amount of nitrogen application and brown rice protein nitrogen in the four periods showed a monotonicity Parabolic relationship, the reason is that excessive nitrogen fertilizer to brown rice production and brown rice protein content decreased.