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花叶海棠通常则多采用叶插繁殖,叶片自扦插约需100天左右,每一个叶片一般仅可得苗1—6株,因而繁殖数不高。 1983年我们采用叶片离体组织培养。取植株上一年生左右的叶片,剪下后用自来水冲洗干净,用75％酒精进行灭菌,再在无菌条件下用无菌水冲洗5—6次,然后将叶片切成长0.5—1厘米的方形小块,放在培养瓶中进行培养。基本培养基为改良MS1附加BA(6-苄基嘌呤)1AA(吲哚乙酸)。培养基的PH在灭菌前用1NN20H调至6,把培养基分装在三角瓶中,在15磅/平方时压下灭菌20分钟,培养温度为24—26℃,每天照光10—12小时,光照强
Mosaic Begonia usually use more leaf plug propagation, leaf cutting since about about 100 days, each leaf is generally available only 1-6 plants, and therefore the number of breeding is not high. In 1983 we used leaf tissue culture in vitro. Take the annual leaves of plants on the annual cut off washed with tap water, sterilized with 75% alcohol, and then washed in sterile conditions with sterile water 5-6 times, and then cut the leaves 0.5-1 cm long Small square, cultured in flasks. The minimal medium was modified MS1 with BA (6-benzylpurine) 1AA (indole acetic acid). The pH of the medium was adjusted to 1 with 1N 20H before sterilization and the medium was dispensed in a Erlenmeyer flask and sterilized at 15 psi for 20 minutes at a temperature of 24-26 ° C and a daily light exposure of 10-12 Hours, light and strong