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用枯草杆菌强启动子P43构建了大肠杆菌-枯草杆菌穿梭表达载体pYG43,其可在大肠杆菌胞内高效表达β-半乳糖苷酶(最高达9200U).利用该载体分泌表达了枯草杆菌碱性纤维素酶(最高达60U/ml),在原宿主菌(B.subtilis 1A747)的基础上提高了5～7倍.
穿梭表达载体pYG43的构建及碱性纤维素酶基因的分泌表达
【摘 要】
：
用枯草杆菌强启动子P43构建了大肠杆菌-枯草杆菌穿梭表达载体pYG43,其可在大肠杆菌胞内高效表达β-半乳糖苷酶(最高达9200U).利用该载体分泌表达了枯草杆菌碱性纤维素酶(最高
【机 构】
：
西北农林科技大学动物科技学院,湖北省武汉阳光广济医药开发有限公司
【出 处】
：
安徽农业科学
【发表日期】
：
2004年期
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