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目的:探讨CCR9/CCL25通路对鼻咽癌CNE-2细胞增殖的影响。方法:培养CNE-2细胞,在培养体系中加入不同浓度的CCL25(0,10,25,50和100ng/ml),用MTT法检测各组细胞的增殖率,确定促CNE-2细胞增殖的最佳CCL25浓度。然后在培养体系中同时加入不同浓度的anti-CCR9(0,0.25,0.5,1,2μg/ml),观察此时CNE-2细胞的增殖情况。结果:不同浓度的CCL25均能促进CNE-2细胞的增殖(P<0.05)。而且其促细胞增殖作用呈现浓度依赖性,50ng/ml时达到最高水平。Anti-CCR9能阻断CCL25的促CNE-2增殖作用(P<0.05),而且也呈浓度依赖性。结论:CCL25具有促进CNE-2细胞增殖的作用,并呈现浓度依赖性。Anti-CCR9能阻断CCL25的促CNE-2增殖作用,并呈浓度依赖性。
Objective: To investigate the effects of CCR9 / CCL25 pathway on the proliferation of nasopharyngeal carcinoma CNE-2 cells. Methods: CNE-2 cells were cultured in vitro. Different concentrations of CCL25 (0, 10, 25, 50 and 100 ng / ml) were added to the culture system. The proliferation rate of each group was determined by MTT assay. The best CCL25 concentration. Then, different concentrations of anti-CCR9 (0, 0.25, 0.5, 1 μg / ml) were simultaneously added to the culture system to observe the proliferation of CNE-2 cells at this time. Results: Different concentrations of CCL25 could promote the proliferation of CNE-2 cells (P <0.05). And its role in promoting cell proliferation showed a concentration-dependent, reaching the highest level of 50ng / ml. Anti-CCR9 blocked CCL25-induced proliferation of CNE-2 cells (P <0.05), but also in a concentration-dependent manner. Conclusion: CCL25 can promote the proliferation of CNE-2 cells in a concentration-dependent manner. Anti-CCR9 blocked CCL25-induced proliferation of CNE-2 in a concentration-dependent manner.