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试验采用旋转回归设计方法，从１９８６～１９９４年在代表松嫩平原黑土区的东北农业大学试验地内进行了以农肥、化肥、秸秆、绿肥等培肥物质为试验因素的长期定位试验。试验分两个阶段进行，前一阶段为培肥阶段，到１９９２年结束。后一阶段为耗竭阶段，主要观测各培肥处理对土壤养分的持续效应及对产量的影响。建立了耗竭阶段农肥、化肥、秸秆对土壤有机质、全氮、全磷及产量的数学模型。随着耗竭试验的继续，各处理土壤磷库含量都呈下降趋势。在农肥、化肥、秸秆三因素中，农肥对土壤磷库的贡献最佳，化肥次之，秸秆最差
The experiment uses the method of rotation regression design. From 1986 to 1994, a long-term experiment was carried out in Northeast Agricultural University on the northeastern agricultural university on the Songnen Plain black soil to test the fertilizers including fertilizer, fertilizer, straw and green manure. The experiment was conducted in two stages, the previous stage being the stage of fertilization and ending in 1992. The latter stage is the depletion stage, the main observation of the fertilization treatment of soil nutrients continued effect and impact on yield. The mathematic model of soil organic matter, total nitrogen, total phosphorus and yield in the depletion stage was established. With the continuation of the depletion test, the contents of soil phosphate pools in all treatments showed a downward trend. Among the three factors of agricultural fertilizer, chemical fertilizer and straw, the contribution of agricultural fertilizer to soil phosphorus pool is the best, followed by chemical fertilizer and the straw is the worst