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AIM:To study the effect of cyclin G2 on proliferation of gastricadenocarcinoma cell line-SGC-7901 cell in vitro.METHODS:By use of cation lipofectamine transfection reagent,the pIRES-G2 and pIRESneo plasmids were transferred intoSGC-7901cell line.Anticlones were selected by G418.Positiveclones were observed and counted using Giemsa staining.Cell proliferative ability was assayed by MTT.RESULTS:(1)The done number of pIRES-G2 group decreased,clone volume reduced.The number of cell clones in pIRESneogroup was 87±3,that of pIRES-G2 group was 53±4,occupying 60.1% of pIRESneo group,there was significantdifference obviously (P<0.01,t=15.45).(2)The averageabsorbance of clone cell obtained by stable transfection ofpIRES-G2 at 570 nm was 1.6966±0.2125,the averageabsorbance of clone cell obtained by stable transfection ofpIRESneo at 570 nm was 2.1182±0.3675,there wassignificant difference between them (P<0.01,t=3.412).CONCLUSION:Cyclin G2 can inhibit SGC-7901cell proliferativeability obviously,it may be a negative regulator in cell cycleregulation.
AIM: To study the effect of cyclin G2 on proliferation of gastricadenocarcinoma cell line-SGC-7901 cell in vitro. METHODS: By use of cation lipofectamine transfection reagent, the pIRES-G2 and pIRESneo plasmids were transferred into SGC-7901 cell line. by G418.Positiveclones were observed and counted using Giemsa staining. Cell proliferative ability was assayed by MTT.RESULTS: (1) The done number of pIRES-G2 group decreased, clone volume reduced.The number of cell clones in pIRESneogroup was 87 ± 3 , that of pIRES-G2 group was 53 ± 4, occupying 60.1% of pIRESneo group, there was significant difference obviously (P <0.01, t = 15.45). (2) The averageabsorbance of clone cell obtained by stable transfection of pIRES-G2 at 570 nm was 1.6966 ± 0.2125, the average absorbance of clone cell obtained by stable transfection of PRESneo at 570 nm was 2.1182 ± 0.3675, there wassignificant difference between them (P <0.01, t = 3.412) .CONCLUSION: Cyclin G2 can inhibit SGC-7901 cell proliferativeability , it may be a negative regulator in cell cycle regulation.