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以盐酸黄连素(berberine-Cl)为模板分子,硅胶为牺牲载体,甲基丙烯酸(MAA)或4-乙烯基吡啶(4-VP)、二甲基丙烯酸乙二醇酯(EDMA)及偶氮二异丁腈(AIBN)分别为功能单体、交联剂及引发剂制备了黄连素印迹聚合物。用光学显微镜观察了聚合物形貌,红外光谱(IR)研究了印迹聚合物(Mip)对模板分子的再结合性能。静态吸附法及Scatchard分析研究了模板在聚合物上的结合特性。结果表明,以MAA为功能单体制备的黄连素印迹聚合物对模板分子有较高的再结合能力,其选择亲和特性来源于聚合物基体中大量在大小、形状及功能基诸方面与模板相匹配的键合位点。由Scatchard图得到了聚合物中两类结合位点的平衡离解常数(Kd)和最大吸附量(qmax)。
Using berberine-Cl as a template and silica gel as the sacrificial carrier, MAA or 4-VP, EDMA and azo Bis (isobutyronitrile) (AIBN) were functional monomers, crosslinkers and initiators of berberine imprinted polymers. The morphology of the polymer was observed by light microscopy. The infrared (IR) spectroscopy was used to investigate the rebondability of Mip to the template. Static adsorption and Scatchard analysis of the template in the polymer binding characteristics. The results showed that the berberine imprinted polymer prepared with MAA as a functional monomer has a high rebondability to the template molecule. The selective affinity characteristics of the berberine imprinted polymer are derived from a large amount of polymer matrix in terms of size, shape and function, Matching bonding sites. The equilibrium dissociation constants (Kd) and maximum adsorption capacity (qmax) of the two types of binding sites in the polymer were obtained from the Scatchard plot.