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本文比较了在包虫病人循环抗原检测中,数种血清不同处理方法。结果证实,在检测包虫病人循环抗原时,预先用胰蛋白酶消化可以使血清免疫复合物中的循环抗原较彻底地游离。用3.5%聚乙二醇沉淀血清中的免疫复合物,再以1%胰蛋白酶消化,较不处理组敏感性提高6倍,较传统的酸解组增加4倍多。另外,将处理血清的稀释液中氯化钠浓度加大,或将血清用10%牛血清白蛋白孵育,可明显降低本底值,P/N值可分别提高1.2倍和1.3倍。
This article compares the detection of circulating antigen in hydatid disease patients, several serum different treatment methods. The results confirm that in the detection of hydatid disease patients circulating antigen, pre-tryptic digestion can make serum immune complexes in the circulating antigen more completely free. The immune complexes were precipitated in 3.5% polyethylene glycol and then digested with 1% trypsin. The sensitivity of the assay was 6-fold higher than that of the untreated group, which was four times more than that of the traditional acid-hydrolysis group. In addition, increasing the concentration of sodium chloride in the dilutions of the treated sera or incubation of the sera with 10% bovine serum albumin significantly reduced the background, with P / N values increased by 1.2 and 1.3, respectively Times