论文部分内容阅读
目的研究各种有丝分裂原诱导的Ｌｙ－ＣＬ反应动力学特征和参与ＣＬ反应的细胞类型。方法从正常人外周血分离ＰＢＭＣ、淋巴细胞和Ｍｏ，经各种有丝分裂原诱导，测定和比较ＰＢＭＣ、淋巴细胞和Ｍｏ的ＣＬ反应强度及动力学。结果ＣｏｎＡ、ＰＨＡ、ＳＰＡ和ＰＷＭ均能诱导淋巴细胞产生ＣＬ反应，其最适刺激浓度分别为：ＣｏｎＡ１００μｇ／ｍｌ、ＰＨＡ１００μｇ／ｍｌ、ＳＰＡ１２５μｇ／ｍｌ、ＰＷＭ＞８００μｇ／ｍｌ。用平皿粘附法去除ＰＢＭＣ中的Ｍｏ，对Ｌｙ－ＣＬ反应无明显影响。Ｍｏ本身对ＣｏｎＡ只能产生缓慢、微弱的ＣＬ反应，而且其动力学与Ｌｙ－ＣＬ具有显著性差异。结论淋巴细胞是有丝分裂原诱导的ＰＢＭＣ－ＣＬ反应的主要产生细胞，Ｌｙ－ＣＬ测定是一种简便、快速检测淋巴细胞早期活化的有效方法。
Objective To investigate the kinetic characteristics of various mitogen-induced Ly-CL reactions and the cell types involved in CL reactions. Methods Peripheral blood mononuclear cells (PBMCs), lymphocytes and Mo were isolated from normal human peripheral blood and induced by various mitogen. The intensity and kinetics of CL reaction between PBMC, lymphocytes and Mo were measured and compared. Results ConA, PHA, SPA and PWM all induced CL responses in lymphocytes. The optimal stimulating concentrations were ConA100μg / ml, PHA100μg / ml, SPA125μg / ml and PWM> 800μg / ml. Removal of Mo in PBMC by plate adhesion method had no significant effect on Ly-CL reaction. Mo itself can only produce slow, weak CL response to ConA, and its kinetics have significant difference with Ly-CL. Conclusion Lymphocytes are the main producing cells of mitogen-induced PBMC-CL response. Ly-CL assay is a simple and rapid method to detect the early activation of lymphocytes.