论文部分内容阅读
Objective:To explore the relationship betweenIL-1β,IL-1Ra gene polymorphism and the occurrence of polycystic ovary syndrome(PCOS) infertility.Methods:A total of59PCOS infertility cases visiting the reproductive center of our hospital fromMar.2010 toMar.2012 and56 healthy women were selected.ELISA method was used for the detection ofIL-1β,IL-1Ra levels, and the levels of serum supersensitivityCreaction protein(US-CRP), insulin(FINS), follicule-stimulating hormone(FSH) and fasting blood-glucose(FBG) were detected.PCR analysis technology was adopted to detect the gene polymorphism of the511 site ofIL-1β and the second introne ofIL-1Ra.Results:The levels ofIL-1β,IL-1Ra,US-CRP,FINS andFBG in blood serum of patients inPCOS group were significantly higher than those in control group(P<0.05 orP<0.01).The level ofFSH inPCOS group was significantly lower than that in control group(P<0.05).The genotypic frequency ofT/T, the511 site ofIL-1β inPCOS group was42.37%, significantly higher than 12.50% in control group(P<0.01).The frequency ofT allele was also significantly higher than that in control group(P<0.01).The genotypic frequency ofⅠ/Ⅴ, the second introne ofIL-1Ra inPCOS group was20.34%, signiciantly higher than3.57% in control group(P<0.05).The frequency of Ⅴ allele inPCOS group was significantly higher than that in control group(P<0.05).Conclusions:T allele of the511 site ofIL-1β gene and Ⅴ allele of the second introne ofIL-1Ra gene might be the genetic basis of the rising ofIL-1β,IL-1Ra andUS-CRP levels in blood serum ofPCOS patients, and are associated with the infertility occurrence ofPCOS patients.