报道了文山松毛虫质型多角体病毒杀虫剂的研制 ,包括多角体的纯化、辅助剂的筛选、剂型研制、产品包装以及病毒杀虫剂的产品质量检测及生产等。该杀虫剂为乳剂 ,多角体浓度达 2 .5×10 9PIB/mL ;所选辅助剂取材方便、容易配制 ,对环境没有污染。经安全检测证明 ,无致病菌 ,符合国家卫生标准 ,对试验动物小白鼠无毒性和致病性。生物测定用 1× 10 6PIB/mL感染 2龄文山松毛虫幼虫 ,其死亡率平均为 85 .5 % This paper reports the development of Dendrolimus punctatus polyclonal virus insecticide, including the purification of polyhedra, the screening of adjuvants, the formulation development, product packaging and the detection and production of virus pesticides. The insecticide is an emulsion with a polyhedron concentration of 2.5 × 10 9 PIB / mL. The selected adjuvants are convenient to obtain, easy to formulate and have no pollution to the environment. After the safety test proved that no pathogens, in line with national health standards, non-toxic and pathogenic test animals in mice. Bioassay The 2-year-old Dendrolimus sinicus larvae were infected with 1 × 10 6 PIB / mL and the average mortality was 85.5%