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采用ACh敏感电极测定相应脊神经节(DRG)内ACh活度的方法,观察切断大鼠前根及电针刺激对相应脊神经节内ACh释放的影响。结果:①切断大鼠左侧L4前根后第3d,切断侧L4DRG内ACh相对含量较切断前相对照侧L4DRG内ACh相对含量明显降低(均为P<0.01)。②切断左侧L4前根前给予电针刺激后,两侧L4DRG内ACh的相对含量较电针前明显增加(P<0.01)。③切断左侧L4前根后第3d给予电针刺激,切断侧L4DRG内ACh相对含量同电针前相比无显著性差异(P>0.05),但显著低于对照侧电针后的ACh相对含量(P<0.01)。提示:DRG内有ACh的释放,ACh的释放是由前根投射到DRG内的胆碱能神经纤维所致。表明在DRG水平前角运动神经元对一级感觉传入实施调控的可能性是存在的。
ACh sensitive electrode was used to measure the activity of ACh in the corresponding spinal ganglia (DRG). The effect of anterior root and electroacupuncture on ACh release in the corresponding spinal ganglia was observed. Results: (1) The relative content of ACh in L4DRG on the severed side was significantly lower than that on the L4DRG on the opposite side (P <0.01). (2) The relative content of ACh in L4DRG on both sides increased significantly compared with those before electroacupuncture (P <0.01). (3) Electro-acupuncture was given on the 3rd day after cutting off the left anterior root of L4, and the relative content of ACh in the L4DRG on the cut side was not significantly different from that before EA (P> 0.05), but significantly lower than that of ACh Content (P <0.01). Tip: ACh release within the DRG, ACh release is caused by the anterior root projection into the cholinergic DRG nerve fibers. Suggesting that there is a possibility that motor neurons at the DRG level modulate primary afferent afferent regulation.