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本研究以抗虫水稻恢复系9311(Bt)及其杂交种F1(Bt)为研究材料,以非转基因的9311为阴性对照,利用ELISA方法研究9311(Bt)及F1(Bt)各生育时期可溶性总蛋白和Bt蛋白的时空变化规律,为转Bt基因抗虫水稻的安全监管提供科学依据。结果表明:外源基因的导入没有引起水稻组织中可溶性总蛋白含量的明显变化;9311(Bt)的Bt蛋白表达量在整个生长周期的各个部位均高于相应的F1(Bt)植株;同一植株不同组织器官中Bt蛋白表达量为:叶片>胚乳>颖壳及茎秆>根;同一植株不同发育期叶片Bt蛋白的测定结果整体表现为:营养生长阶段>生殖生长阶段>成熟衰老阶段。研究结果为转Bt基因抗虫水稻适宜检测时期的选择提供了一定的参考。
In this study, insect-resistant rice restorer line 9311 (Bt) and its hybrid F1 (Bt) were used as materials. The non-transgenic 9311 was used as a negative control and the soluble forms of 9311 (Bt) and F1 Total protein and Bt protein spatiotemporal variation rules for transgenic Bt insect-resistant rice to provide scientific basis for safety regulation. The results showed that the introduction of exogenous gene did not cause significant changes in soluble total protein content in rice tissues. The expression of Bt protein in 9311 (Bt) was higher than that in F1 (Bt) plants in all parts of the whole growth cycle. The expression of Bt protein in different tissues and organs was: leaf> endosperm> glume and stalk> roots. The results of the determination of Bt protein in different developmental stages of the same plant showed as a whole: vegetative growth stage> reproductive growth stage> mature aging stage. The results provide a reference for the selection of transgenic Bt transgenic rice suitable for the detection period.