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目的研究新型选择性雌激素受体调节剂(SERMs)xy9902对大鼠心肌重构的干预作用,并初步探讨其机制。方法腹主动脉缩窄复制压力负荷增高致大鼠心肌重构动物模型,术后2周存活动物分为模型组(AAC)、卡托普利组(Cap)及xy9902组(xy9902),另设假手术组(Sham)。Mal-lory三色染色观察心肌组织胶原纤维含量的变化。黄嘌呤氧化酶法测定心肌组织SOD活性,硫代巴比妥酸法测定MDA含量。结果与Sham组比较,AAC组心脏指数HMI及LVMI增加,心肌细胞排列紊乱、增粗,细胞间隙明显增宽,可见大量异常胶原纤维堆积,CVF增大,然而Cap组和xy9902组明显好转。AAC组心肌SOD活性下降,MDA含量增加,Cap组和xy9902组与AAC组比较,SOD活性增加,MDA含量下降(P<0.01)。相关分析结果表明CVF与SOD/MDA比值呈负相关(P<0.01)。结论 xy9902对心肌重构有防治作用,其对体内氧化应激平衡的正向调控可能是抗心肌重构的分子机制之一。
Objective To study the intervention of new selective estrogen receptor modulator (sERMs) xy9902 on myocardial remodeling in rats and its mechanism. Methods The rat model of cardiac remodeling was induced by the constriction of abdominal aorta, and the surviving animals were divided into model group (AAC), cap and xy9902 group (xy9902) Sham operation group (Sham). Mal-lory trichrome staining of myocardial tissue collagen content changes. The activity of SOD in myocardial tissue was measured by xanthine oxidase method and MDA content was measured by thiobarbituric acid method. Results Compared with Sham group, cardiac index (HMI) and LVMI increased, cardiomyocytes were disordered and thickened in AAC group, and cell gap was obviously widened. A large number of abnormal collagen fibers accumulated and CVF increased, however, Cap group and xy9902 group improved obviously. Compared with AAC group, SOD activity and MDA content in Cap group and xy9902 group were increased (P <0.01). Correlation analysis showed a negative correlation between CVF and SOD / MDA (P <0.01). Conclusion xy9902 has a preventive and therapeutic effect on myocardial remodeling, and its positive regulation on the balance of oxidative stress in vivo may be one of the molecular mechanisms of anti-myocardial remodeling.