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目的:分别建立超微粉和细粉三妙丸和四妙丸的HPLC指纹图谱,并进行对比研究。方法:用HPLC色谱法,对不同粉体形态的妙丸建立指纹图谱,采用“中药色谱指纹图谱相似度评价软件”建立标准指纹图谱,对不同粉体形态各妙丸的指纹图谱进行相似度分析。结果:方法的重复性及稳定性良好。两种不同粉体形态的妙丸指纹图谱的相似度均大于0.90,两者在共有峰上没明显差别,而峰面积有差别。结论:该方法稳定准确,建立的HPLC指纹图谱,能从整体上反映出三妙丸和四妙丸的化学成分信息,为建立质量标准提供了参考依据。
OBJECTIVE: To establish the HPLC fingerprints of superfine powder and fine powder Sanmiao Wan and Si Miaowan, respectively, and compare them. Methods: HPLC fingerprint was used to establish the fingerprint of Miao Wan with different powder morphology. The fingerprint of each Miao pill with different powder morphology was established by using the “Fingerprint Similarity Evaluation Software for TCM Chromatography” to establish the standard fingerprints. Degree analysis. Results: The repeatability and stability of the method are good. The similarity of the wonderful pill’s fingerprint of two different powder forms are all greater than 0.90, there is no significant difference between the two shared peaks, but the peak area is different. Conclusion: The method is stable and accurate. The established HPLC fingerprinting can reflect the chemical composition information of Sanmiao Wan and Si Miaowan as a whole and provide a reference for establishing the quality standard.