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目的 :观察中药复方四毒清对脂多糖 (LPS)攻击小鼠生存率和器官功能的影响 ,寻找有效中药防治LPS性多器官功能衰竭。方法 :将中药四毒清按常规方法煎制成 1kg/L药液 ,以小鼠为实验对象 ,灌胃给药 ,3d后腹腔注射LPS(6 0mg/kg) ,观察小鼠生存率。另分批进行同样实验 ,观察小鼠血清丙氨酸转氨酶 (ALT)活性、血清肌酐(Cr)和尿素氮 (BUN)含量及肺、肝、肾、肠的病理变化。结果 :LPS注射后 96h ,LPS组小鼠的死亡率为 73% (n =34) ,而四毒清防治组小鼠的死亡率为 35 % (n =31) ,两组死亡率有明显差别 (P <0 0 5 )。同时 ,LPS组小鼠的血清ALT活性、Cr和BUN含量明显高于对照组 ,而四毒清防治组小鼠血清ALT活性、Cr和BUN含量明显低于LPS组。病理学检查发现四毒清防治组小鼠肺、肾及小肠病变明显轻于LPS组。结论 :中药四毒清能防治LPS性多器官功能障碍 ,提高LPS攻击小鼠的生存率。
Objective: To observe the effect of Siduqing, a traditional Chinese medicine compound, on the survival rate and organ function of mice challenged with lipopolysaccharide (LPS), and to find effective Chinese medicines for preventing and treating LPS multi-organ failure. Methods : The traditional Chinese medicine Teijinqing was made into a 1kg/L liquid by conventional method. The mice were used as the experimental subjects and administered intragastrically. Three days later, LPS (60 mg/kg) was injected intraperitoneally to observe the survival rate of the mice. The same experiment was performed in separate batches to observe the serum alanine aminotransferase (ALT) activity, serum creatinine (Cr) and urea nitrogen (BUN) levels, and pathological changes in lung, liver, kidney, and intestine. RESULTS: At 96 h after LPS injection, the mortality rate of mice in the LPS group was 73% (n = 34), whereas the mortality rate in the Siduqing prevention group was 35% (n = 31). There was a significant difference in mortality between the two groups. (P < 0 0 5). At the same time, the serum ALT activity, Cr and BUN levels in LPS group mice were significantly higher than those in the control group, while the serum ALT activity, Cr and BUN contents in the Siduqing prevention group mice were significantly lower than those in the LPS group. Pathological examination found that the lung, kidney, and small intestine lesions in the Siduqing prevention group were significantly lighter than those in the LPS group. Conclusion : Traditional Chinese medicine Tetramidine can prevent and treat LPS multiple organ dysfunction and improve the survival rate of LPS challenge mice.