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以3-氨基苯硼酸为单体,通过化学沉积的方法在L-半胱氨酸修饰过的金膜表面合成了针对血红蛋白(hemoglobin,Hb)的分子印迹聚合物膜(molecularly imprinted polymer film,MIF),并作为表面等离子共振传感器(SPR)的识别单元.优化模板Hb同单体的比例,并对该条件下制备的MIF表面形貌及厚度进行表征.优化MIF吸附条件,实验结果表明MIF修饰的SPR传感器对Hb具有较高的灵敏度和吸附能力,该条件下所能检测到的Hb最低浓度值为0.005 mg/mL.选择性和稳定性实验结果表明,MIF对牛血清蛋白、溶菌酶和卵清蛋白具有良好的选择效果,同时具有良好的稳定性.尿液配制Hb样品的检测结果显示该传感器在较为复杂的环境中对Hb仍具有良好的响应.
Using 3-aminophenylboronic acid as monomer, a molecularly imprinted polymer film (MIF) on hemoglobin (Hb) was synthesized by chemical deposition on the surface of L-cysteine modified gold membrane. ) As the identification unit of the surface plasmon resonance sensor (SPR), the ratio of template Hb to monomer was optimized, and the surface morphology and thickness of MIF prepared under this condition were optimized.MIF adsorption conditions were optimized and the experimental results showed that MIF modification Of the SPR sensor has a high sensitivity and adsorption ability to Hb, the lowest concentration of Hb detected under this condition is 0.005 mg / mL.Selectivity and stability The experimental results show that MIF on bovine serum albumin, lysozyme and Ovalbumin has a good selection effect and good stability.Results of the urine preparation Hb sample show that the sensor still has a good response to Hb in the more complicated environment.