论文部分内容阅读
目的研究醛固酮合成酶(CYP11B2)基因-344T/C多态性和血压、血糖、血脂相互作用与急性脑梗死(ACI)易感性的关系。方法采用病例对照研究方法,检测研究对象的血压、血糖和血脂情况,采用聚合酶链反应限制性内切酶长度多态性(PCR-RFLP)方法检测CYP11B2的基因型。结果病例组CYP11B2基因TC和CC基因型的频率显著高于对照组,分别为38.12%和9.97%;相对于TT基因型,暴露于TC和CC基因型人群的OR值分别为1.72和1.88。病例组C等位基因的频率也显著高于对照组,为29.03%;相对于T等位基因,C等位基因的OR值为1.57。此外研究还显示CYP11B2基因TC或CC基因型与血糖、血脂相互作用可增加ACI的易感性,而该基因型与血压无协同作用。结论当个体携带TC或CC基因型时,ACI的易感性增加,该两种基因型与血糖、血脂相互作用,可增加ACI的易感性。
Objective To investigate the relationship between -344T / C polymorphism of aldosterone synthase (CYP11B2) gene and blood pressure, blood glucose, blood lipid and the susceptibility to acute cerebral infarction (ACI). Methods The case-control study was used to detect the blood pressure, blood glucose and blood lipids in the study subjects. The genotypes of CYP11B2 were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Results The frequencies of TC and CC genotypes in CYP11B2 gene were significantly higher in case group than those in control group (38.12% and 9.97%, respectively). Compared with TT genotype, the odds ratio of exposure to TC and CC genotypes were 1.72 and 1.88, respectively. The frequency of C allele in case group was also significantly higher than that in control group, which was 29.03%. The OR of C allele was 1.57 with respect to T allele. In addition, the study also showed that CYP11B2 gene TC or CC genotypes and blood glucose, lipid interactions can increase the susceptibility of ACI, and the genotype has no synergistic effect with blood pressure. Conclusion When the individual carries TC or CC genotype, the susceptibility of ACI is increased. The interaction of these two genotypes with blood sugar and blood lipids can increase the susceptibility of ACI.