论文部分内容阅读
目的:探讨川芎嗪对大肠癌实体瘤及其血管生成的抑制作用与机制。方法:建立大肠癌sw620裸鼠移植瘤模型,随机分成5组:生理盐水组,川芎嗪低、中、高3个剂量组及恩度组。给药后检测移植瘤的体积和质量,观察移植瘤的病理形态学改变,并分别用免疫组化法和Western blotting法检测移植瘤组织中CD34、VEGF、HIF-1α蛋白表达。结果:与生理盐水组相比,川芎嗪中、高剂量组大肠癌sw620移植瘤的体积和质量明显减小,其瘤体内CD34、VEGF、HIF-1α的表达明显降低。结论:川芎嗪能抑制大肠癌sw620裸鼠移植瘤的生长,其作用机制可能与改善肿瘤组织的乏氧状况、抑制肿瘤血管生成有关。
Objective: To investigate the inhibitory effect and mechanism of ligustrazine on solid tumor and its angiogenesis in colorectal cancer. Methods: The transplanted tumor model of large intestine sw620 nude mice was established and randomly divided into 5 groups: normal saline group, low, medium and high doses of ligustrazine and Ende group. The volume and the mass of the xenografts were detected after the administration, and the pathomorphological changes of the xenografts were observed. The expressions of CD34, VEGF and HIF-1α in the xenografts were detected by immunohistochemistry and Western blotting respectively. Results: Compared with the saline group, the volume and the mass of the sw620 xenografts in the medium and high doses of ligustrazine were significantly decreased, and the expressions of CD34, VEGF and HIF-1α in the tumor tissues were significantly decreased. CONCLUSION: Tetramethylpyrazine can inhibit the growth of transplanted tumor of sw620 in nude mice with colorectal cancer. Its mechanism may be related to the improvement of hypoxia status and inhibition of tumor angiogenesis.