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以杜梨根瘤为试材,采用分子生物学方法、牛津杯法和指示植物接种法,研究了杜梨根癌病原菌的质粒类型及枯草芽孢杆菌对杜梨根癌病原菌的影响。结果表明:从1年生杜梨根部冠瘿瘤上分离、纯化、培养菌株,得到4株与根癌病原菌相似菌株,对之进行PCR扩增,此4株菌株均获得特异性目的条带,鉴定为根癌土壤杆菌胭脂碱类型。将病原菌接种指示植物向日葵幼苗,4株菌株均有致病性。经室内离体试验,供试的9株枯草芽孢杆菌对病原菌表现不同程度的抑菌效果;接种指示植物试验中,菌株9076和9161过滤发酵液能够完全抑制冠瘿瘤的生长。
Using the pear root nodule as test material, the type of plasmid and the effect of Bacillus subtilis on the pathogenic bacteria of Pear Root were studied by molecular biology method, Oxford cup method and indicator plant inoculation method. The results showed that four isolates of the same pathogenic bacteria were isolated, purified and cultivated from the root of Coriolus versicolor. The four strains were identified by PCR For Agrobacterium tumefaciens nopaline type. The pathogen inoculated plant sunflower seedlings, four strains have pathogenicity. Nine strains of Bacillus subtilis were tested for their antibacterial effect on pathogenic bacteria by in vitro tests. In the inoculated plants, strains 9076 and 9161 were able to completely inhibit the growth of the tumor.