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目的:探讨重组蜂毒肽作用于大鼠C6胶质瘤细胞后,对细胞生长的抑制作用及对血管内皮生长因子(VEGF)、血管内皮生长因子受体(Flt-1)、基质金属蛋白酶-9(MMP-9)表达的影响。方法:对大鼠C6胶质瘤细胞进行体外培养后,分为对照组及重组蜂毒肽低、中、高剂量治疗组,治疗组分别给予相应浓度的重组蜂毒肽(20,50,80 mg·L-1),采用MTT法于24,48,72 h分别测定C6胶质瘤细胞的生长情况;并采用Western blot法测定不同浓度重组蜂毒肽作用72 h后C6胶质瘤细胞VEGF,Flt-1,MMP-9的表达情况。结果:与对照组比较,不同浓度的重组蜂毒肽作用于细胞24 h后,细胞生长情况无明显变化,但48,72 h后,低、中、高浓度治疗组的细胞的增殖活性均有不同程度的降低、生长抑制率增高(P<0.01或P<0.05);同时中、高浓度重组蜂毒肽作用72 h后,胶质瘤细胞的VEGF,Flt-1,MMP-9表达水平均有不同程度的降低(P<0.01或P<0.05)。结论:重组蜂毒肽对C6胶质瘤细胞的生长和VEGF,Flt-1,MMP-9的表达均有明显的抑制作用,因此重组蜂毒肽可能通过抑制VEGF,Flt-1,MMP-9的表达来抑制胶质瘤的生长。
AIM: To investigate the inhibitory effect of recombinant melittin on rat C6 glioma cells and its effect on the expression of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor (Flt-1), matrix metalloproteinase- 9 (MMP-9) expression. Methods: C6 glioma cells were cultured in vitro and divided into control and recombinant melittin low, medium and high dose treatment groups, the treatment group were given the corresponding concentration of recombinant melittin (20,50,80 mg · L-1). The growth of C6 glioma cells was determined by MTT assay at 24, 48 and 72 h respectively. Western blot was used to determine the expression of VEGF in C6 glioma cells after 72 h treatment with different concentrations of recombinant melittin , Flt-1, MMP-9 expression. Results: Compared with the control group, the cell growth did not change after treated with different concentrations of recombinant melittin for 24 h, but after 48 and 72 h, the proliferation activity of cells in low, medium and high concentration groups (P <0.01 or P <0.05). At the same time, the expression levels of VEGF, Flt-1 and MMP-9 in glioma cells after medium and high concentrations of recombinant melittin for 72 h There are different degrees of reduction (P <0.01 or P <0.05). Conclusion: Melittin can significantly inhibit the growth of C6 glioma cells and the expression of VEGF, Flt-1 and MMP-9, so the recombinant melittin may inhibit the expression of VEGF, Flt-1, MMP-9 Of the expression to inhibit glioma growth.