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针对红脂大小蠹危害程度不同的3个地区的球孢白僵菌种群,利用ISSR(inter‐simple sequence repeat)分子标记分析了各个种群的遗传多样性。从19条引物中筛选出10条多态性高、稳定性好的ISSR引物用于扩增分析。68株球孢白僵菌的Nei’s基因多样性(h)为0.2973,Shannon指数(Is)为0.4488。旬邑、宜君、古交3地白僵菌种群间的基因分化系数(GST)为0.0525,基因流(Nm)为9.0255;而来源于土壤、红脂大小蠹、蛀屑和树皮的白僵菌种群间的基因分化系数为0.1449,基因流为2.9508。各球孢白僵菌种群表现出不同的多样性水平,旬邑种群和红脂大小蠹虫种群的遗传多样性相对较高;地理分布种群间的差异不如分离基质种群间的差异明显,地理分布种群间存在明显的基因交流,而分离基质种群间的基因流较低,遗传分化明显。
According to the populations of Beauveria bassiana in three regions with different degrees of harm, the inter-simple sequence repeat (ISSR) markers were used to analyze the genetic diversity of each population. Nineteen ISSR primers with high polymorphism and good stability were screened from 19 primers for amplification analysis. The 68 strains of Beauveria bassiana Nei’s gene diversity (h) was 0.2973, Shannon index (Is) 0.4488. The genetic differentiation coefficient (GST) and the gene flow (Nm) of Beauveria bassiana in Xunyi, Yijun and Gujiao were 0.05255 and 9.0255, respectively, while those derived from soil, Betula platyphylla, The coefficient of genetic differentiation between Beauveria bassiana populations was 0.1449 and the gene flow was 2.9508. Beauveria bassiana populations showed different diversity levels, and the genetic diversity of Xunyi population and Red-bellied beetle populations was relatively high. The differences among the geographical distribution populations were not as obvious as that between the separated matrix populations, and the geographical distribution There was obvious gene exchange among the populations, while the gene flow between the separated matrix populations was low and the genetic differentiation was obvious.