用胶原酶消化和体外培养的方法成功地获得了大量有活性和较纯的新生猪胰岛细胞，用微囊包膜技术包裹胰岛细胞，并进行培养。由放射免疫药盒对培养液中胰岛素的含量测定、胰岛素释放试验和组织学检查等体外实验所得的数据，证实了微囊和非微囊的新生猪胰岛细胞的活性和分泌功能的差异不显著，说明本包膜材料和一步法制囊新技术对胰岛细胞无损伤，微囊包膜后胰岛细胞的活性和分泌功能良好。 A large number of active and pure neonatal porcine islets were successfully obtained by collagenase digestion and in vitro culture. The pancreatic islet cells were encapsulated by microencapsulation and cultured. The results of in vitro experiments such as determination of insulin content, insulin release test and histological examination in radioimmunoassay kit showed that there was no significant difference in the activity and secretion of islets of newborn porcine islets between non-microcapsules and non-microcapsules , Indicating that the envelope material and one-step bag making technology has no damage to islet cells, and the activity and secretion function of islet cells after microcapsule encapsulation are good.