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本文采用免疫细胞化学方法研究了16～38周人胎儿初级树皮质含Calbindin神经元的发育．16周时，含Calbindin神经元主要分布于脑室带、脑室下带和中间带深部，其他皮质带散在存在。脑室带和脑室下带的细胞大多跑体较大，胞浆和胞核均含阳性产物，而其它皮质带细胞胞核常不染色。19周时，脑室带和脑室下带的含Cal－bindin神经元明显减少，仅存在极少量，其它皮质带含Calbindin神经元的状况类似16周。21周时大多数含Cal－bindin神经元位于Ⅴ、Ⅵ层。以后，随着服龄增大，较多的含Calbindin神经元逐渐依次出现于Ⅳ、Ⅲ和Ⅱ层，遵循皮质神经元从深至浅的发育规律。这些神经元的形态也由以双极细胞为主发育成以多极细胞为主，染色逐渐加深，突起逐渐增长。在19～28周，皮质Ⅰ层的一些Cajal-Retzius细胞和水平纤维亦表达Calbindin。本实验中较多的含Calbindin神经元于发育早期在皮质增殖带的出现，提示Calbindin在皮质的早期发育过程中可能发挥重要作用。
In this paper, immunocytochemistry was used to study the development of Calbindin neurons in the primary bark of human fetus from 16 to 38 weeks. At 16 weeks, Calbindin-containing neurons were mainly distributed in the ventricular zone, subventricular zone and medial zone with deep, other cortex scattered. Most of the cells in the ventricular zone and subventricular zone run large, with positive products in the cytoplasm and nucleus, while the nuclei of other cortical cells are often not stained. At 19 weeks, the number of Cal-bindin-containing neurons in the ventricular and subventricular zone was significantly reduced with only a very small amount, similar to that of other cortex with Calbindin neurons. At 21 weeks, most Cal-bindin-containing neurons were located in the Ⅴ and Ⅵ layers. Later, with the increase of service age, more Calbindin-containing neurons gradually appeared in the layers Ⅳ, Ⅲ and Ⅱ, following the cortical neurons from deep to shallow development. The morphology of these neurons also dominated by bipolar cells to multipolar cells, staining gradually deepened, the protrusions grow. At 19-28 weeks, some of the Cajal-Retzius cells and horizontal fibers in the cortical I layer also expressed Calbindin. In our experiment, more Calbindin-containing neurons appeared in the cortical proliferating zone early in development, suggesting that Calbindin may play an important role in the early development of cortex.