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目的:观察丹参酮ⅡA(Tan ⅡA)对低氧培养下人胃癌SGC7901细胞增殖、凋亡及HIF-1α表达的影响.方法:用氯化钴(CoCl2)创建低氧模型,设常氧对照组、低氧对照组和低氧加不同浓度Tan ⅡA组.分别取0.5、1.0、2.0、5.0、10.0mg/L的Tan ⅡA干预低氧胃癌SGC7901细胞24、48、72h后,MTT法检测细胞活力;用上述同样浓度的Tan ⅡA干预低氧胃癌细胞48h和72h后,HOECHST染色法检测细胞凋亡.Tan ⅡA(0.5、2.0、10.0mg/L)干预低氧胃癌细胞48h后,免疫细胞化学二步法检测HIF-1α蛋白表达的变化.结果:MTT法证实,低氧条件下,Tan ⅡA呈时间、剂量依赖性地抑制胃癌SGC-7901细胞增殖(P<0.01),10.0mg/LTan ⅡA作用细胞72h后,其抑制率达71.2%.HOECHST染色法发现,低氧条件下,分别用0.5-10.0mg/L浓度的Tan ⅡA作用胃癌细胞48、72h,Tan ⅡA可呈时间、剂量依赖性地诱导胃癌SGC-7901细胞凋亡(F=60354.00,187922.10,均P<0.05),10.0mg/LTan ⅡA作用细胞72h后,凋亡率达40.70%±1.55%.免疫细胞化学法显示,Tan ⅡA呈剂量依赖性地抑制低氧诱导的HIF-1α蛋白表达(F=712.326,P<0.01).结论:低氧条件下,Tan ⅡA抑制胃癌细胞增殖并诱导其凋亡,这种作用可能与其抑制HIF-1α蛋白表达有关.
Objective: To observe the effects of TanⅡA on the proliferation, apoptosis and the expression of HIF-1α in human gastric cancer cell line SGC7901 under hypoxic culture.Methods: Hypoxia model was established with cobalt chloride (CoCl2) Hypoxia control group and hypoxia plus different concentrations of Tan Ⅱ A group.The cell viability was detected by MTT assay after Tan Ⅱ A were treated with Tan Ⅱ A at a dose of 0.5,1.0,2.0,5.0,10.0mg / L for 24,48,72h respectively, Hypoxic gastric cancer cells were treated with the same concentration of Tan Ⅱ A for 48h and 72h, HOECHST staining was used to detect the apoptosis.Tan ⅡA (0.5, 2.0, 10.0mg / L) intervention of hypoxic gastric cancer cells for 48h, (HIF-1α) in gastric cancer cell line SGC-7901 was detected by MTT assay.Results: MTT assay showed that Tan ⅡA inhibited the proliferation of gastric cancer cell SGC-7901 in a time-and dose-dependent manner under hypoxic conditions (P <0.01) After 72h, the inhibitory rate was 71.2% .HuECHST staining showed that under the condition of hypoxia, TanⅡA was treated with Tan ⅡA at concentration of 0.5-10.0mg / L for 48 and 72h, respectively. Tan IIA was induced in a time-and dose-dependent manner The apoptosis of gastric cancer SGC-7901 cells (F = 60354.00, 187922.10, all P <0.05), and the effect of 10.0mg / , And the apoptotic rate was 40.70% ± 1.55% .Immunocytochemistry showed that TanⅡA inhibited hypoxia-induced HIF-1α protein expression in a dose-dependent manner (F = 712.326, P <0.01) .Conclusion: Under hypoxic conditions , Tan Ⅱ A inhibited gastric cancer cell proliferation and induced apoptosis, which may be related to the inhibition of HIF-1α protein expression.