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目的 研究我国G1型轮状病毒主要中和抗原VP7基因的变异特点。方法 对 1988～1998年收集自北京、沈阳、新乡、上海、深圳、广州、重庆等 7个城市的 2 3份G1型轮状病毒毒株VP7cDNA序列进行测定 ,并结合核酸电泳带型和G1型单抗的ELISA检测结果分析VP7基因的变异特点。结果 我国G1型轮状病毒VP7变异有一定规律 ,主要集中在aa41、49、5 7、65、68、74、94、97、147、170、2 17、2 18、2 68、2 81、2 91,即VR3～ 5、VR7～ 8以及多肽C端的部分区域内。各地区之间没有明显差别 ,虽然随时间不同个别氨基酸可能发生替换 ,但仍同属Jpn 417支系 ;aa91位可能参与构成G1型VP7的抗原表位 ,某些位点的氨基酸变异可能与核酸带型改变有关。结论 在研制疫苗时 ,应采用基因和抗原性具有代表性的近期毒株 ,同时定期监测抗原性的改变
Objective To study the variation of VP7 gene of major neutralizing antigen of G1 rotavirus in China. Methods The sequences of 23 strains of VP7 cDNA of rotavirus type G1 that were collected from Beijing, Shenyang, Xinxiang, Shanghai, Shenzhen, Guangzhou, Chongqing and other seven cities from 1988 to 1998 were determined. The results of ELISA showed that the VP7 gene mutation characteristics. Results The variation of VP7 of the G1 rotavirus in our country has some laws, mainly concentrated in aa41,49,5 7,65,68,74,94,97,147,170,2 17,218,268,281,2 91, namely VR3 ~ 5, VR7 ~ 8 and C-terminal part of the polypeptide region. There is no significant difference between the regions. Although some individual amino acids may be replaced with time, they belong to Jpn 417. Aa91 may be involved in the epitope forming G1 type VP7. Amino acid variation at some sites may be related to nucleic acid Type change related. Conclusions In the development of vaccines, a representative recent strain of genes and antigens should be used, with regular monitoring of antigenic changes