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目的:研究自体骨髓和脐带血有核细胞治疗肌萎缩侧索硬化(ALS)的疗效。方法:采集1例ALS患者的自体骨髓或新生儿脐带血,获取含干细胞的有核细胞悬液,每份平均含有核细胞4.72×108个。通过腰椎穿刺,将其移植入该患者的蛛网膜下腔。在4年内分别对本例ALS患者进行骨髓及新生儿脐带血有核细胞移植治疗3次和50次,观察患者治疗后运动功能的变化,脑脊液常规、生化指标和不良反应。结果:第1次自体骨髓移植后病情好转,第3次移植后达到最好状态。此后患者病情逐渐加重,但较移植治疗前进展速度明显减缓。治疗过程中患者脑脊液压力、常规生化指标均在正常范围内,无明显不良反应。结论:自体骨髓和脐带血有核细胞移植治疗ALS有短期效果,可延缓ALS的进展,该方法是安全的。
Objective: To study the efficacy of autologous bone marrow and umbilical cord blood cells in treating amyotrophic lateral sclerosis (ALS). Methods: One patient with autologous bone marrow or neonatal umbilical cord blood was collected from patients with ALS to obtain nucleated cell suspension containing stem cells, each containing an average of 4.72 × 108 nuclear cells. Through the lumbar puncture, it was transplanted into the patient’s subarachnoid space. The patients with ALS were treated with bone marrow and neonatal umbilical cord blood cell transplantation three times and 50 times respectively within 4 years. The changes of motor function, cerebrospinal fluid routine, biochemical indexes and adverse reactions were observed. Results: After the first autologous bone marrow transplantation, the condition improved and the best condition was achieved after the third transplantation. Since then the patient’s condition gradually aggravated, but significantly slower than the rate of progress of the transplant treatment. During the treatment of cerebrospinal fluid pressure in patients with conventional biochemical indicators are in the normal range, no significant adverse reactions. CONCLUSION: Autologous bone marrow and umbilical cord blood cell transplantation for the treatment of ALS has a short-term effect, which can delay the progression of ALS. This method is safe.