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一、材料1.IIE染液:苏木素2g,纯乙醇100nd,甘油100ml,蒸馏水100Inl,钾明矾15g,冰醋酸10Inl,碘酸钠300IDg,沉淀酸化伊红Y6g。将苏木素溶于乙醇中,钾明矾用蒸馏水加热溶解,混合二液,然后加人甘油、碘酸钠、冰醋酸,最后加伊红。2.稀氨水溶液:用立式染色缸量取5()
First, the material 1. IIE dye: hematoxylin 2g, pure ethanol 100nd, glycerol 100ml, distilled water 100Inl, potassium alum 15g, glacial acetic acid 10Inl, sodium iodide 300IDg, precipitation acidification Eosin Y6g. The hematoxylin dissolved in ethanol, potassium alum with distilled water heated to dissolve, mixed two liquid, then add glycerin, sodium iodate, glacial acetic acid, and finally add yihong. 2. Dilute ammonia solution: with a vertical dyeing cylinder measuring 5 ()