论文部分内容阅读
采用花粉发育单核期的‘锦丰’梨花药,接种在1/2 MS附加IAA 0.2mg/L、BA1～2mg/L的培养基上,经过120天产生胚状体。胚状体转入MS附加GA3 0.1mg/L、IBA 0.2mg/L、BA 1mg/L的分化培养基上,经过85天分化幼梢。无根植株转入1/2 MS附加IAA 1.5mg/L的培养基上,经过14天诱导生根,移栽和嫩枝嫁接成活情况良好。
The ’Jinfeng’ pear anthers with pollen development mononuclear stage were inoculated into 1/2 MS medium supplemented with IAA 0.2 mg / L and BA1 ~ 2 mg / L to produce embryoid body after 120 days. Embryoid bodies were transferred to differentiation medium with MS supplemented with GA3 0.1 mg / L, IBA 0.2 mg / L, and BA 1 mg / L, and the young shoots were differentiated after 85 days. Rooted plants were transferred to 1/2 MS supplemented with IAA 1.5 mg / L medium, rooting was induced after 14 days, and transplanting and shoot grafting survived well.