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目的 :探讨益气化瘀方对椎间盘软骨终板内血管状态的影响 ,了解椎间盘营养供应的情况。方法 :选择 8月龄清洁级 SD雄性大鼠 5 0只。选择 5月对照组、5月模型组为本实验观察时段。大鼠随机分为对照组、模型组 ,益气化瘀方、活血通络方、芬必得组 ,每组 1 0只 ,雌雄各半。体视学分析、Dissector自动计数分析软骨终板内血管芽的数量、面积 ;免疫组化检测血管内皮生长因子 ( VEGF)在软骨终板的表达情况 ,进行VEGF与血管芽之间的相关性分析。结果 :益气化瘀方可以增加血管芽的数量和面积 ,与活血通络方作用相似 ,优于芬必得 ;模型组软骨终板内 VEGF表达明显 ,但表达率减少 ;益气化瘀方 VEGF表达量降低 ,但表达率增加明显。VEGF的表达与血管芽密度有正相关。结论 :益气化瘀方可以增加血管芽数量和 VEGF的表达 ,促进血管修复、再生和增加椎间盘营养供应的作用。
Objective : To investigate the effect of Yiqi Huayu Decoction on the vascular status of the endplate of the intervertebral disc and to understand the nutritional status of the intervertebral disc. Methods : A total of 50 male clean SD rats aged 8 months were selected. The May control group and the May model group were selected as the experimental observation period. Rats were randomly divided into control group and model group, Yiqi Huayu Fang, Huoxuetongluo Recipe, and Fenbid group. Each group had 10 rats, male and female. Stereological analysis, dissector automatic counting analysis of the number and area of vascular buds in cartilage endplates; immunohistochemistry detection of vascular endothelial growth factor (VEGF) expression in cartilage endplates, and correlation analysis between VEGF and vascular buds . Results: Yiqi Huayu Recipe can increase the number and area of vascular buds, which is similar to that of Huoxuetongluo recipe and better than Fenbid. The expression of VEGF in the cartilage endplates of the model group is obvious, but the expression rate is decreased; Yiqi Huayufang VEGF The expression level decreased, but the expression rate increased significantly. The expression of VEGF is positively correlated with the density of blood vessel buds. Conclusion: Yiqi Huayu Recipe can increase the number of vascular buds and the expression of VEGF, promote the repair and regeneration of blood vessels and increase the nutritional supply of intervertebral discs.