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目的探讨过氧化小体增殖物激活受体γ(PPARγ)C161T基因多态性与动脉粥样硬化性脑梗死(ACI)的关系。方法筛选168例ACI患者及165名健康人群为研究对象。采用聚合酶链式限制性片段长度多态性方法检测PPARγ基因C161T位点多态性。结果 PPARγC161T基因型CC、CT和TT在脑梗死患者组的分布频率分别为76.2%、21.4%和2.4%,C等位基因频率为86.9%,T等位基因频率为13.1%;CC、CT和TT基因型在正常对照组中的分布频率分别为60.6%、36.4%和3.0%,C等位基因频率为78.8%,T等位基因频率为21.2%,除TT基因型外(例数少未统计分析),两组间其他各基因型频率及等位基因频率差异有显著性,正常对照组T等位基因携带者基因型(CT+TT)频率(39.4%)明显高于脑梗死组(23.8%),CC型明显低于脑梗死组。结论 PPARγC161T基因多态性与脑梗死的发病有关,T等位基因携带者可能发生脑梗死的风险低。
Objective To investigate the relationship between C161T gene polymorphism of peroxisome proliferator - activated receptor γ (PPARγ) and atherosclerotic cerebral infarction (ACI). Methods 168 patients with ACI and 165 healthy subjects were selected as the study subjects. Polymorphism of C161T gene of PPARγ gene was detected by polymerase chain reaction-restriction fragment length polymorphism. Results The distribution frequency of CC, CT and TT in patients with cerebral infarction was 76.2%, 21.4% and 2.4% respectively, the frequency of C allele was 86.9% and the frequency of T allele was 13.1% The frequency of TT genotype in normal controls were 60.6%, 36.4% and 3.0%, respectively. The frequency of C allele was 78.8% and the frequency of T allele was 21.2%. In addition to the TT genotype Statistical analysis showed that there was significant difference in the frequencies of other genotypes and alleles between the two groups. The frequency of CT + TT (39.4%) in T allele of the normal control group was significantly higher than that in the cerebral infarction group 23.8%), CC type was significantly lower than that of cerebral infarction group. Conclusion Polymorphism of PPARγC161T gene is associated with the pathogenesis of cerebral infarction. T allele carriers may have a lower risk of cerebral infarction.