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目的探讨我国湖北汉族人Toll样受体(TLR)4基因Asp299Gly和CD14 C-260T基因多态性分布与大肠癌的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测110例大肠癌患者及160例正常对照者TLR4基因Asp299Gly及CD14 C-260T基因型及等位基因频率的分布。结果大肠癌组CD14 C-260T基因型与正常对照组比较,差异有统计学意义(P＜0．05)。正常对照组CC基因型的频率为15．6%,明显低于大肠癌组的31．8%(P=0．0027,OR=0．3968,95%CI=0．2209～0．7129)；正常对照组中CT基因型的频率为48．1%,明显高于大肠癌组的30．9% (P=0．0056,OR=2．074,95%CI=1．246～3．462)。所有样本中均未发现TLR4基因Asp299Gly的突变型。结论CD14 C-260T基因多态性与中国湖北汉族大肠癌显著相关,而TLR4基因Asp299Gly多态性与大肠癌无关。
Objective To investigate the association between the polymorphisms of Toll-like receptor (TLR) 4 gene Asp299Gly and CD14 C-260T gene and colorectal cancer in Hubei Han population in China. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the distribution of TLR4 gene Asp299Gly and CD14 C-260T genotypes and allele frequencies in 110 patients with colorectal cancer and 160 normal controls. . Results There was significant difference in CD14 C-260T genotype between colorectal cancer group and normal control group (P<0.05). The frequency of CC genotype in the normal control group was 15.6%, which was significantly lower than that of the colorectal cancer group (31.8%, P=0.0027, OR=0.3968, 95% CI=0.2209 to 0.7129). The frequency of CT genotype in the normal control group was 48.1%, which was significantly higher than that of the colorectal cancer group (30.9%) (P=0.0056, OR=2.074, 95%CI=1.246-3. 462). No mutation in the TLR4 gene Asp299Gly was found in all samples. Conclusion The CD14 C-260T gene polymorphism is significantly associated with colorectal cancer in Chinese Han population in Hubei, but the TLR4 gene Asp299Gly polymorphism is not associated with colorectal cancer.