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目的 探讨天花粉蛋白 (trichosanthin ,TCS)对荷人肝癌裸小鼠的抑癌作用。方法 采用人肝癌原位移植模型。将 2 4只裸小鼠分为TCSⅠ组、TCSⅡ组、TCSⅢ组和对照组 ,TCS剂量分别为 0 2mg/kg、0 4mg/kg、0 6mg/kg ,对照组用生理盐水 0 2ml/只腹腔注射 ,共 10次。第 2 1天称瘤重和裸小鼠体重 ,计算抑癌率。应用免疫组织化学技术 ,检测瘤组织中Ki6 7的表达。结果 对照组、TCSⅠ组、TCSⅡ组和TCSⅢ组平均瘤重分别为 0 99g、0 81g、0 2 2 g和 0 2 0g。用药组瘤重与对照组相比有显著性差异 (P <0 0 5 )。TCSⅠ组、TCSⅡ组和TCSⅢ组抑癌率分别为 18 18%、77 78%、79 80 %。对照组、TCSⅠ组、TCSⅡ组和TCSⅢ组平均鼠重增加为 2 2 2 g、1 2 2 g、0 2 8g、- 2 37g。用药组与对照组鼠重变化有显著性差异 (P <0 0 5 )。对照组、TCSⅠ组、TCSⅡ组和TCSⅢ组Ki6 7蛋白阳性表达百分率分别为 31 33%、2 9 92 %、10 2 5 %和 9 6 7%。TCSⅠ组Ki6 7百分率与对照组相比无显著性差异 (P >0 0 5 )。TCSⅡ组和TCSⅢ组Ki6 7百分率与对照组相比有显著性差异 (P <0 0 5 )。结论 TCS对荷人肝癌裸小鼠的移植瘤有明显的抑癌作用。TCS抑制肿瘤细胞增殖可能是其抑癌的机制之一。
Objective To investigate the antitumor effect of trichosanthin (TCS) on human hepatocellular carcinoma in nude mice. Methods Human orthotopic liver transplantation model was used. 24 nude mice were divided into TCSⅠgroup, TCSⅡgroup, TCSⅢgroup and control group. The TCS doses were 0 2mg / kg, 0 4mg / kg and 0 6mg / kg, respectively. The control group was treated with saline 0 2ml / Injection, a total of 10 times. On the 21st day, tumor weight and nude mice were weighed and the tumor suppression rate was calculated. Immunohistochemistry was used to detect the expression of Ki67 in tumor tissue. Results In the control group, the mean tumor weights of TCSⅠ, TCSⅡ and TCSⅢ groups were 0 99g, 0 81g, 0 2 2 g and 0 2 0g, respectively. The tumor weight of the treatment group compared with the control group had significant difference (P <0 05). The antitumor rates of TCSⅠ, TCSⅡ and TCSⅢ were 18 18%, 77 78% and 79 80% respectively. In the control group, the mean rat weights in the TCSⅠ, TCSⅡ and TCSⅢ groups were 2222g, 122g, 028g and -237g, respectively. There was a significant difference in the rat weight between the drug group and the control group (P <0.05). In the control group, the positive expression rates of Ki6 7 protein in TCSⅠ, TCSⅡ and TCSⅢ groups were 31 33%, 292%, 102 5% and 96 7% respectively. The percentage of Ki6 7 in TCSⅠgroup was not significantly different from that in control group (P> 0.05). The percentage of Ki6 7 in TCSⅡgroup and TCSⅢgroup was significantly higher than that in control group (P <0.05). Conclusion TCS has obvious anti-tumor effect on the xenografts of nude mice bearing hepatocellular carcinoma. TCS inhibition of tumor cell proliferation may be one of its mechanisms of tumor suppressor.