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目的:构建稳定、可靠的小鼠唯支持细胞综合征模型。方法:60只SPF级NIH小鼠分成两组,分别给予30 mg/kg白消安腹腔注射及睾丸冷冻(0℃)30、60 min处理。于处理后2、4及8周,观察小鼠的生存率、睾丸重量及生精功能的变化,并对精子发生障碍程度进行定量化分析。结果:各组小鼠的基础体重值及处理后小鼠的生存率均无明显差异(P>0.05)。白消安处理后第4及8周,睾丸重量(g)(4周:0.04±0.01;8周:0.05±0.01)均显著低于对照组(4周:0.09±0.03;8周:0.11±0.02)(P<0.05),生精小管呈“唯支持细胞综合征”样改变,Johnsen评分(4周:3.86±0.50;8周:2.70±0.67)较对照组(4周:9.60±0.25;8周:9.76±0.43)均显著降低(P<0.01);睾丸冷冻30及60 min后,第2、4及8周睾丸重量(g)(冷冻30 min,2周:0.07±0.02;4周:0.06±0.01;8周:0.09±0.01)(冷冻60 min,2周:0.05±0.01;4周:0.04±0.02;8周:0.04±0.02)显著低于同期对照侧(2周:0.11±0.01;4周:0.11±0.01;8周:0.12±0.00)(P<0.05),睾丸形态学上易见生精小管萎缩、脱落、坏死及纤维化。各期冷冻组Johnsen评分(冷冻30 min,2周:4.70±0.67;4周;2.70±0.84;8周:6.10±1.14;冷冻60 min,2周:1.67±0.58;4周:1.20±0.45;8周:1.00±0.00)均显著低于同期对照组(2周:9.60±3.23;4周:9.60±0.55;8周:9.70±0.45)(P<0.01)。结论:白消安处理对小鼠的生存率无明显影响,可用来制备稳定、可靠的小鼠唯支持细胞综合征模型。
Objective: To construct a stable and reliable mouse model of cell-only syndrome. Methods: Sixty SPF NIH mice were divided into two groups and given 30 mg / kg busulfan intraperitoneally and testicular freezing (0 ℃) for 30 and 60 min respectively. At 2, 4 and 8 weeks after treatment, the changes of survival rate, testicular weight and spermatogenesis in mice were observed, and the degree of spermatogenesis was quantitatively analyzed. Results: The body weight of mice in each group and the survival rate of mice after treatment showed no significant difference (P> 0.05). Testis weight (g) (4 weeks: 0.04 ± 0.01; 8 weeks: 0.05 ± 0.01) was significantly lower at 4 and 8 weeks after busulfan treatment (4 weeks: 0.09 ± 0.03; 8 weeks: 0.11 ± 0.02) (P <0.05). The number of seminiferous tubules was changed to “only supportive cell syndrome”, the Johnsen score was significantly higher than that of the control group (4 weeks: 3.86 ± 0.50; 0.25 and 8 weeks: 9.76 ± 0.43) (P <0.01). Testicular weight (g) at weeks 2, 4 and 8 after freezing for 30 and 60 min (freezing for 30 min and 2 weeks: 0.07 ± 0.02; 4 weeks: 0.06 ± 0.01; 8 weeks: 0.09 ± 0.01) (frozen 60 min, 2 weeks: 0.05 ± 0.01; 4 weeks: 0.04 ± 0.02; 8 weeks: 0.04 ± 0.02) 0.11 ± 0.01; 4 weeks: 0.11 ± 0.01; 8 weeks: 0.12 ± 0.00) (P <0.05). Testicular tubule atrophy, shedding, necrosis and fibrosis were easily observed in testis. The Johnsen score of each frozen group (frozen 30 min, 2 weeks: 4.70 ± 0.67; 4 weeks; 2.70 ± 0.84; 8 weeks: 6.10 ± 1.14; frozen 60 min, 2 weeks: 1.67 ± 0.58; 4 weeks: 1.20 ± 0.45; 8 weeks: 1.00 ± 0.00) were significantly lower than the control group (2 weeks: 9.60 ± 3.23; 4 weeks: 9.60 ± 0.55; 8 weeks: 9.70 ± 0.45) (P <0.01). Conclusion: Busulfan has no obvious effect on the survival rate of mice, which can be used to prepare a stable and reliable mouse model of cell-only syndrome.