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采用线粒体(mtDNA)控制区序列分析方法,进行了条石鲷养殖群体(F1代和F2代)与野生群体的遗传变异比较研究。结果表明:在长度为484bp的mtDNA控制区片段中,条石鲷养殖群体F1代单倍型多样度水平(h=0.955±0.047)略低于野生群体(h=1.000±0.034),却显著高于养殖F2代群体(h=0.797±0.070);条石鲷野生群体(π=0.021±0.012)、养殖群体F1代(π=0.018±0.010)和养殖群体F2代(π=0.014±0.008)的核苷酸多样度水平呈现明显的递减趋势;核苷酸不配对分布和两两序列相比较碱基差异结果显示条石鲷野生群体、养殖群体F1代和养殖群体F2代也呈现明显的递减趋势。分子方差分析(AMOVA)、两两群体相比较的ФST和确切P检验结果皆显示条石鲷养殖群体F1代和F2代与野生群体存在显著的遗传分化。单倍型最小跨度分析和NJ系统分析结果均未检测到显著的谱系结构。
A comparative study on the genetic variation of the grouper (F1 and F2) and wild populations collected from the grouper (mitochondria) control region sequence analysis method was carried out. The results showed that in the mtDNA control region with a length of 484bp, the haplotype diversity level (F1) was significantly lower (h = 0.955 ± 0.047) than the wild population (h = 1.000 ± 0.034), but significantly higher In the F2 population (h = 0.797 ± 0.070), the percentage of the wild group (π = 0.021 ± 0.012), the F1 generation (π = 0.018 ± 0.010) and the F2 generation Nucleotide diversity showed a significant decreasing trend; nucleotide unpaired distribution and base sequence differences compared to two base sequence results showed that the wild snipeheadfish population, breeding population F1 generation and breeding population F2 also showed a significant downward trend . Molecular variance analysis (AMOVA), ФST in comparison with the two groups and the exact P test showed that there was a significant genetic differentiation between F1 generation and F2 generation of barnospora group and wild populations. Haplotype minimum span analysis and NJ system analysis results did not detect significant lineage structure.