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目的比较两种经典方麻杏石甘汤(MXSGT)和银翘散(YQS)抗流感病毒药理特点。方法采用细胞病变法(CPE)和MTT法检测麻杏石甘汤和银翘散体外抑制流感病毒的作用;q PCR法和ELISA法检测病毒感染后诱导细胞因子及趋化因子分泌。结果麻杏石甘汤体外对不同亚型流感病毒(甲型流感H1N1、H6N2、H9N2等亚型及乙型流感)的半数有效浓度(IC50)在0.1~1 mg·mL~(-1)之间,银翘散体外未显示抑制流感病毒的作用。与病毒对照组比较,麻杏石甘汤(3 mg·mL~(-1))和银翘散(0.5 mg·mL~(-1))体外均抑制流感病毒诱导细胞产生细胞因子及趋化因子的表达,包括IL-6、IP-10、TNF-α、IL-8、CCL5(P<0.05),其中麻杏石甘汤对IP-10、IL-6的下调作用更显著(P<0.05)。结论对两个经典治疗流感的复方比较发现,麻杏石甘汤兼具直接抗病毒和抑制病毒介导炎症的作用,其作用机制有待后续实验进一步验证。银翘散仅显示了抗炎药效。
Objective To compare the pharmacological characteristics of two classic prescriptions MXSGT and YQS. Methods Cytopathic effect (CPE) and MTT assay were used to detect the effect of Maxingshigan Decoction and Yinqiaosan on influenza virus in vitro. Q PCR and ELISA were used to detect the cytokines and chemokines secretion induced by virus infection. Results The median effective concentration (IC50) of Maxingshigan Decoction in different subtypes of Influenza A (Influenza A H1N1, H6N2, H9N2 and other subtypes and Influenza B) in 0.1-1 mg · mL -1 Room, Yin Qiao scattered outside the body does not show the role of influenza virus. Compared with the virus control group, Maxingshigan Decoction (3 mg · mL -1) and Yinqiao Powder (0.5 mg · mL -1) inhibited the cytokine production and chemotaxis induced by influenza virus in vitro The expression of IL-6, IP-10, TNF-α, IL-8 and CCL5 were significantly decreased in Maxing Shigan Decoction (P < 0.05). Conclusion Comparing the two classic therapeutic flu comparisons found that Maxing Shigan Decoction both direct antiviral and inhibit virus-mediated inflammation, the role of mechanism to be further verified by subsequent experiments. Yinqiaosan only shows anti-inflammatory efficacy.