论文部分内容阅读
为了解引起河北省苹果再植病害的病原菌,在河北省10个地区苹果园中采集土壤样品,在实验室进行病原菌的诱集分离培养,根据形态和分子特征对主要病原菌进行种类鉴定。结果表明,在分离得到的293株真菌中,有116株镰孢菌,为分离频率最高的真菌。在形态学鉴定的基础上,对供试镰孢菌进行了分子鉴定。在基于核糖体基因内转录间隔区(rDNA‐ITS)序列与翻译延长因子1α(EF‐1α)序列片段构建的系统发育树中,代表菌株分别与GenBank登记的所属菌株聚于同一群。研究结果明确了河北省苹果再植病害的疑似致病镰孢菌,包括:尖孢镰孢Fusarium oxysporum、木贼镰孢F.equiseti、锐顶镰孢F.acuminatum、层出镰孢F.proliferatum和茄腐镰孢F.solani。
In order to understand the pathogen causing apple replantation disease in Hebei Province, soil samples were collected from apple orchards in 10 areas of Hebei Province. Pathogenic bacteria were isolated and cultured in the laboratory, and the main pathogens were identified according to morphological and molecular characteristics. The results showed that of the 293 isolated fungi, 116 Fusarium species were the most frequently isolated fungi. On the basis of morphological identification, Fusarium graminearum was molecularly identified. In the phylogenetic tree based on the rDNA-ITS sequence and the fragment of the elongation factor 1α (EF-1α) sequence, the representative strains were clustered with the corresponding GenBank-deposited strains. The results of the study clearly identified the suspected pathogenic Fusarium of apple replantation diseases in Hebei Province, including Fusarium oxysporum, F. equiseti, F. acuminatum, F.proliferatum And F. solani.