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【目的】分析转拟南芥△1-吡咯啉-5-羧酸合成酶(P5CS1)基因羽衣甘蓝的耐盐性,为获得较强的耐盐性羽衣甘蓝品种及其抗逆育种提供理论依据。【方法】将拟南芥P5CS1基因(AtP5CS1)经农杆菌介导转入羽衣甘蓝植物中,在盐胁迫下,分别检测转基因植株与野生型植株的AtP5CS1 mRNA表达量、幼苗脯氨酸含量、株系根系性状、整株干质量和鲜质量、叶片相对水含量、叶片电导率和整株存活率。【结果】在150 mmol/L NaCl胁迫下,转基因植株的P5CS1基因mRNA可正常表达,与对照相比,转基因株系Y1、Y2的主根和最长侧根长度较长,侧根数目较多,整株干质量和鲜质量较重;而且相对水含量显著高于对照植株(P<0.05,下同),脯氨酸含量及存活率均极显著高于对照植株(P<0.01),叶片相对电导率显著低于对照植株。【结论】转AtP5CS1基因植株的耐盐表型优于对照,即AtP5CS1基因在羽衣甘蓝中的表达明显改善了转基因植株的耐盐性。
【Objective】 The purpose of this study was to analyze the salt-tolerance of the transgenic P5CS1 gene, Kale, in order to provide a theoretical basis for obtaining strong salt-tolerant Kale varieties and their anti-retrograde breeding . 【Method】 Arabidopsis thaliana P5CS1 gene (AtP5CS1) was transferred into Kale plants via Agrobacterium tumefaciens. Under salt stress, the expression of AtP5CS1 mRNA, proline content, Department of root traits, the whole plant dry weight and fresh quality, leaf relative water content, leaf conductivity and whole plant survival rate. 【Result】 Under the stress of 150 mmol / L NaCl, P5CS1 mRNA of transgenic plants could be expressed normally. Compared with the control, the main roots and longest lateral roots of transgenic lines Y1 and Y2 had longer length and more lateral roots, (P <0.05, the same below), proline content and survival rate were significantly higher than the control plants (P <0.01), the relative conductivity of leaves Significantly lower than the control plants. 【Conclusion】 The salt tolerant phenotype of AtP5CS1 transgenic plants was better than that of the control. The expression of AtP5CS1 gene in Kale significantly improved the salt tolerance of transgenic plants.