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目的:建立HPLC法测定雅静胶囊中厄贝沙坦和氢氯噻嗪的含量。方法:色谱柱:Dianmonsil~(TM) C_(18)柱(250mm×4.6 mm,5μm);流动相:乙腈-0.08mol·L~(-1)磷酸溶液(用三乙胺调至pH5.0)(40:60);流速:1ml·min~(-1);紫外检测波长:225nm;进样量:20μl;柱温:25℃。结果:厄贝沙坦和氢氯噻嗪分别在15～135μg·ml~(-1)(r=0.999 9)和1.2～10.8μg·ml~(-1)(r=0.999 9)浓度范围内线性关系良好,平均回收率分别为100.0%和100.5%。结论:本方法操作简便、快捷,重复性好,灵敏度高,结果准确可靠,适用于雅静胶囊的质量控制。
Objective: To establish an HPLC method for the determination of irbesartan and hydrochlorothiazide in Yajing Capsules. METHODS: The mobile phase consisted of Dianmonsil TM C 18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.08 mol·L -1 phosphoric acid (adjusted to pH 5.0 with triethylamine ) (40:60); flow rate: 1 ml · min -1; UV detection wavelength: 225 nm; injection volume: 20 μl; Results: Irbesartan and hydrochlorothiazide had good linearity in the range of 15 ~ 135μg · ml -1 (r = 0.999 9) and 1.2 ~ 10.8μg · ml -1 (r = 0.999 9) respectively The average recoveries were 100.0% and 100.5%, respectively. Conclusion: The method is simple, rapid, good reproducibility, high sensitivity, accurate and reliable, suitable for Yajing capsule quality control.