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将田间自然发病抗性鉴定法与利用苹果轮纹病菌ZZ26人工接种枝条鉴定法相结合,对189份栽培种苹果种质资源进行了苹果轮纹病综合抗性鉴定,并根据抗病性鉴定结果对其进行抗病类型分类和评价。结果表明:不同苹果种质资源抗病性表现存在差异,但参试种质中未发现免疫种质。通过人工接种,189份苹果种质资源的抗病类型可分为:高抗(16个);中抗(75个);中感(81个);高感(17个)。通过田间调查,2012年得到抗病类型3种,即高抗(144个);中抗(44个);中感(1个)。2013年同样是3个抗病类型:高抗(14个);中抗(162个);中感(13个)。两年调查均无高感品种。2017年再次调查,结果显示田间调查与人工接种鉴定结果有随种植年限增加一致率逐渐提高的趋势,2012年为13.23%,2013年为38.62%,2017年则超过一半,达到57.14%。经过综合抗性筛选,最终得到表现一致性较好的高抗种质2份:北之幸和秦冠。
Combining the identification method of natural resistance in field with the identification method of artificial inoculation branches of ZZ26 in apple germplasm, apple germplasm comprehensive identification of apple germplasm resources was carried out on 189 cultivars of apple germplasm resources. According to the identification results of the disease resistance Its classification and evaluation of disease-resistant types. The results showed that there were differences in the disease resistance of different apple germplasm resources, but no immune germplasm was found in the tested germplasm. By artificial inoculation, the resistance types of 189 apple germplasm resources can be divided into: high resistance (16); medium resistance (75); moderate feeling (81); high sense (17). According to the field survey, three types of disease resistance were obtained in 2012, namely high resistance (144), moderate resistance (44) and moderate infection (1). In 2013, there were also 3 types of disease resistance: high resistance (14); moderate resistance (162); moderate flu (13). Two years of investigation are not highly susceptible varieties. In 2017, the survey again showed that there was a trend of increasing consistent rates of field investigation and artificial inoculation with increasing years of planting. The consistency rate in 2013 was 13.23% in 2012, 38.62% in 2013 and more than half in 2017, reaching 57.14%. After a comprehensive resistance screening, and ultimately get better performance consistent high resistance to germplasm 2: Fortunately North and Qin crown.