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目的:研究大白栓茵乙酸乙酯萃取部位的降糖机制。方法:建立2型糖尿病小鼠模型,选取造模成功的小鼠随机分为4组,分别给予大白栓茵丝体乙酸乙酯萃取部位低、高剂量,二甲双胍和等体积的0.5%CMC-Na溶液。4周后检测各组小鼠血糖、胰岛素、胰岛素敏感性指数,测定肝、肌糖原含量。采用酶联免疫试剂盒测定小鼠血清TNF-α、IL-6、CRP含量,小鼠胰腺组织中TNF-α、IL-6含量。结果:与模型组比较,大白栓茵乙酸乙酯萃取部位能够提高胰岛素敏感性和肝糖原含量(P<0.01),降低糖尿病小鼠血糖、血清IL-6、CRP及胰腺组织中TNF-α、IL-6含量(P<0.01)。结论:大白栓茵乙酸乙酯萃取部位能够降低2型糖尿病小鼠血糖,其降糖机制与改善肝糖原代谢,抑制炎症因子生成,提高胰岛素敏感性有关。
OBJECTIVE: To study the hypoglycemic mechanism of Dabiesin ethyl acetate extract. Methods: A mouse model of type 2 diabetes mellitus was established. Mice that were successful in modeling were randomly divided into 4 groups. The mice were given low, high dose ethyl acetate extract, metformin and equal volume of 0.5% CMC-Na Solution. After 4 weeks, the blood glucose, insulin and insulin sensitivity index of each group were detected, and the contents of liver and muscle glycogen were determined. Serum levels of TNF-α, IL-6 and CRP were measured by enzyme-linked immunosorbent assay (ELISA). The contents of TNF-α and IL-6 in mouse pancreatic tissue were determined. Results: Compared with the model group, ethyl acetate extract of Daphnixin could improve insulin sensitivity and hepatic glycogen content (P <0.01), decrease blood glucose, serum IL-6, CRP and TNF-α in pancreatic tissue , IL-6 content (P <0.01). Conclusion: EbES extract can reduce blood glucose in type 2 diabetic mice, and its hypoglycemic mechanism is related to improving glycogen metabolism, inhibiting the production of inflammatory cytokines and increasing insulin sensitivity.