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目的:探讨白藜芦醇(Res)对人激素非依赖性前列腺癌体外生长细胞PC-3的抑制作用及实现其抑制作用的可能途径。方法:按白藜芦醇浓度分为25,50,100和200 ttmoI/L 4个处理组和对照组(未加白藜芦醇),处理PC-3细胞不同时间后,倒置显微镜观察细胞形态;MTT法检测细胞的增殖抑制情况;免疫组化sP法分析Sur-vivin蛋白的表达。结果:与对照组比较,白藜芦醇处理组可使细胞明显变圆、体积缩小、脱壁细胞增多;MTT法检测显示,白藜芦醇对PC-3细胞的增殖有抑制作用,并呈时间-剂量依赖性,其中24 h组细胞存活率分别为86.2%,77.15%,60.89%和35.76%,48 h组细胞存活率分别为65.24%,59.76%,46.47%和27.77%,白藜芦醇处理组随着浓度的增加和作用时间延长,对PC-3细胞的增殖抑制作用增强,与对照组比较差异有统计学意义(P<0.05);免疫组化结果显示,Survivin蛋白在100 gmol/L白藜芦醇作用下较对照组表达明显减弱(P<0.01)。结论:白藜芦醇对体外生长的PC-3细胞有明显的抑制作用,下调Survivin蛋白表达而诱导前列腺癌细胞凋亡可能是其抑制肿瘤生长的机制之一。
OBJECTIVE: To investigate the inhibitory effect of resveratrol on human prostate cancer cell line PC-3 in vitro and its possible mechanism. Methods: The cells were divided into 4 groups (25, 50, 100 and 200 ttmo I / L) and control group (without resveratrol) according to the concentration of resveratrol. After treated with different concentrations of resveratrol, the cell morphology was observed by inverted microscope. MTT Method to detect cell proliferation inhibition; immunohistochemistry sP method analysis Sur-vivin protein expression. Results: Compared with the control group, the resveratrol treatment group could significantly round the cells, the volume was reduced, and the number of detached cells increased; the results of MTT assay showed that resveratrol inhibited the proliferation of PC-3 cells The cell survival rates in 24 h group were 86.2%, 77.15%, 60.89% and 35.76%, respectively. The cell viability in 48 h group was 65.24%, 59.76%, 46.47% and 27.77% Compared with the control group, the alcohol treatment group showed a significant difference (P <0.05) with the increase of the concentration and the prolongation of the action time, and inhibited the proliferation of PC-3 cells. The results of immunohistochemistry showed that Survivin protein was stable at 100 gmol / L resveratrol significantly decreased compared with the control group (P <0.01). CONCLUSION: Resveratrol significantly inhibits the growth of PC-3 cells in vitro. Down-regulating the expression of Survivin and inducing the apoptosis of prostate cancer cells may be one of the mechanisms of its inhibition of tumor growth.