【摘 要】
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The mechanism of the hydrolysis of ribonucleic acid or oligonucleotide(A)by ribonu-clease A(RNase A)has been extensively in vestigated.According to the opinion
【出 处】
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Acta Chimica Sinica(English Edition)
论文部分内容阅读
The mechanism of the hydrolysis of ribonucleic acid or oligonucleotide(A)by ribonu-clease A(RNase A)has been extensively in vestigated.According to the opinions of pre-vious works,the hydrolytic reaction takes place in two successive steps:(1)transphos-phorylation,i.e.,the formation of ribonucleoside 2’,3’-cyclophosphate(B)accompanied by si-multaneous breaking of the nucleotidyl linkage and(2)hydrolyzation,i.e.,the hydrolysis of B
The mechanism of the hydrolysis of ribonucleic acid or oligonucleotide (A) by ribonu-clease A (RNase A) has been extensively in vestigated. According to the opinions of pre-vious works, the hydrolytic reaction takes place in two successive steps: (1 ) transphos-phorylation, ie, the formation of ribonucleoside 2 ’, 3’-cyclophosphate (B) accompanied by si-multaneous breaking of the nucleotidyl linkage and (2) hydrolyzation, ie, the hydrolysis of B
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